LOCUS EY190537 477 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAB-aaa03c03.b1 R.similis_EST_RSAB Radopholus similis cDNA 3', mRNA sequence. ACCESSION EY190537 VERSION EY190537.1 GI:159492828 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 477) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 915 was extended to value 916 (,) This trace has been recalled with phred original value before phred recall for SL was 55 original value before phred recall for SR was 521 Seq primer: pDNR-LIB-reverse. FEATURES Location/Qualifiers source 1..477 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022112 R.similis_EST_RSAB" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 aggcggccga catgtcccct tcaggggttt tttttttttt tttttgcagt ttggagacct 61 tttaaggggt tcaacgtttc aaggatcatc atcgtttggc aaagagcagt aattgggggt 121 gctgttgctt ttttcgccgg tttcggctgt tcggccccca ctgccaatgt gcgggagcac 181 tcgtgcgatg aagtgctgct gctgctgctg cggcgcttgg gcttctgccg tcggctgctg 241 ctgccgctcc gcacgcccga acccgtcctt cacttcggcg cactcttgca gcagctgctc 301 ctccgaatgg gcgccgacgg cggcaggctc caagtaggcg aactgcagac ccagcattgt 361 ggcgctgttt ggcgctgagc gagtgagcag aaatacagaa atagtatcag tgggatgcgg 421 gttggctcct ctcgggagag ttggcggcgc acaaacaaat gtgtgtcccc ggccgta //