LOCUS       EY190529                 416 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa02a07.b1 R.similis_EST_RSAB Radopholus similis cDNA 3'
            similar to ref|NP_508734.1| Choline kinase (49.2 kD)
            [Caenorhabditis elegans] gb|AAK39220.1| Hypothetical protein
            C52B9.1 [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY190529
VERSION     EY190529.1  GI:159492820
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 847 was extended to value 848 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 27
            original value before phred recall for SR was 438
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttcctt ttgggggttt tttttttttt ttagggctaa tgggaatctt
       61 ttggggagtc attatagtga aaatgttcta gcgtaaaagc aatttatgta aactttaaag
      121 tagataagat ataaattcaa aaccgggtaa ttcttcattg ctggaacttc tctaggagat
      181 gcttgttttt gtagtacaat cccaatcggt cacggccata ttcagcgaat ccaaagtcca
      241 cgggcgaaac ctccatctgg agaagtcccc agacccccca gagcaagtga gacacaggaa
      301 taaacggaat tgtctcctca accatagtct gggcaacatt ttgcaaatgt tcctccgacg
      361 aagaggggtg caactccttc tcgtagttgt acatgaactc tagcaccccg gccgta
//