LOCUS       EY190516                 500 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa02h12.b1 R.similis_EST_RSAB Radopholus similis cDNA 3'
            similar to sp|P27443|MAOM_ASCSU NAD-dependent malic enzyme,
            mitochondrial precursor (NAD-ME), mRNA sequence.
ACCESSION   EY190516
VERSION     EY190516.1  GI:159492807
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 856 was extended to value 857 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 56
            original value before phred recall for SR was 861
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgtccata aggggggttt tttttttttt tttcaaactc ggatcttttt
       61 ggggaggggt tgaatgaatg ttgaatagac ttgctacaat gtgatgatgt ctcgagggga
      121 ttttgactcg gcatataatc taaacgttgt gtcatggttt ttaaacaggg caccggagag
      181 ttaatgaaat aaaaaaacga gacatctagc ataagcacgg ggagatacac cgccccagag
      241 gaaaggatca tctttgcgat attaatatcc attttattga gctattattt agtcgtccat
      301 gctgtcacga cgcaccggtg ggatcggaaa gccatggcgc atgtcctggc tgggccagtc
      361 gtaggtcttg ttgatcaggt catcgtattc gactgagtag acctgggagc ggatgaacat
      421 ttccttgtct tccggttcgg ggtagagctt tgccgtcccg tccctgtagc actcttccgc
      481 cagtttgatg gcaatcttga
//