LOCUS       EY190512                 353 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa02f06.b1 R.similis_EST_RSAB Radopholus similis cDNA 3'
            similar to gb|AAK72124.1| beta-actin [Chrysophrys auratus], mRNA
            sequence.
ACCESSION   EY190512
VERSION     EY190512.1  GI:159492803
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 907 was extended to value 907 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 53
            original value before phred recall for SR was 475
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ctgaaagaag tttccaggca aaaaaatgga agggtggaaa aaaagcactt ttctgttttc
       61 tgtctgtctg ccttcctctg tgtctgtctc gctgtccgct gtccctgctt aaaagcactt
      121 gcggtgcacg atggacgggc ccgactcgtc gtactcctgc ttgctgatcc acatctgctg
      181 gaagggggac agcgaggcca ggatggagcc tccgatccaa acggagtact tgcgctcagg
      241 aggagcgatg atcttgatct tcatcgtcga cggggccaga gcggtgatct ccttctgcat
      301 gcggtcggca attcccgggt acatggtggt gcccccagaa agccccggcc gta
//