LOCUS EY190487 508 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAB-aaa02b02.b1 R.similis_EST_RSAB Radopholus similis cDNA 3' similar to ref|NP_490676.1| Ribosomal Protein, Large subunit RPL-7 (28.1 kD) (rpl-7) [Caenorhabditis elegans] sp|O01802|RL7_CAEEL 60S ribosomal protein L7 pir|T29034 hypothetical protein F53G12.10 - Caenorhabditis elegans gb|AAB54165.1| Ribosomal protein, l, mRNA sequence. ACCESSION EY190487 VERSION EY190487.1 GI:159492931 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 508) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 879 was extended to value 880 (,) This trace has been recalled with phred original value before phred recall for SL was 59 original value before phred recall for SR was 565 COMM possible reversed clone; protein similarity on negative strand Seq primer: pDNR-LIB-reverse. FEATURES Location/Qualifiers source 1..508 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022112 R.similis_EST_RSAB" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 aggcggccga catgttcttt tttggttttt tttttttttt ttttcggttt gagagccctt 61 tttggggatc gaaataacaa aaaatagtgt gacacaaccg gaactccaat cctgggggca 121 agaagtttgt ttatctggtc ctctcggttt ccctagtcac ctccctccac aaagtggttt 181 gacttcttct tgaatccacc gaccgggttg ctcagtttta acggccacaa gaagttggtc 241 accgccttga agtgcggccc gacagtccaa atctggtgga tcatgtcctc tacacagatc 301 acatcccgtt tgccgaggat actctcaaca atgttgttgt ccgtgatggg gatgcgctgt 361 cccttttcct tggcatagcc acgcttgtag accaacatgc ggatgacctt ctggctaggg 421 tagccccacc cgatgtacgg gtcggcgatg cgcatcttct ggatggtggc cttgtttaat 481 tttactaaaa caccgctgtc cccgccgt //