LOCUS       EY190478                 617 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa01b10.b1 R.similis_EST_RSAB Radopholus similis cDNA 3'
            similar to ref|NP_492761.1| Nucleoside diphosphate kinase
            [Caenorhabditis elegans] pir|T21354 hypothetical protein F25H2.5 -
            Caenorhabditis elegans emb|CAB02101.1| Hypothetical protein F25H2.5
            [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY190478
VERSION     EY190478.1  GI:159492922
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 819 was extended to value 820 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 24
            original value before phred recall for SR was 636
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga cctgttccat aagggggttt tttttttttt ttggcaacac aaagtttcct
       61 tttggggact ttaagtttcg acacaataag aataagaaat cctcgaaatc aaatggtctt
      121 gtgaattggt atccgggact ttcttcctta tcgcagatgg gcgtcttcca gattgttcct
      181 cactcgtaga tccagttgat ctgcgctggc tcccagtcaa ccaattcttc ctgcttgaac
      241 cagtgtgcaa tttcacgctc tgccgatgga acactgtctg atccgtggca gatgtttcgt
      301 cccatgtcca tacagtagct tccacgaatt gttcctgctg cactcaccag tggatttgtg
      361 gctccgagca tagctcgacc ttgtttcaca acatcgagtc cttcccaaac cattgccaca
      421 actggccctg aagccatgta cttcactaat ccagcgaaga aaggtttgtc cttcagatcc
      481 tcataatgca cttccaagtg ttggggcgtt gctttgacca ttttgatagc caccagttta
      541 taaccacgct gctcaaattg tttgattatc ttgctcacaa gaccgcgttt ggacaccatc
      601 aggcttgccc ggccgta
//