LOCUS       EY190477                 404 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa01c11.b1 R.similis_EST_RSAB Radopholus similis cDNA 3'
            similar to ref|NP_506513.1| TRNA Methylase TRM-1 (trm-1)
            [Caenorhabditis elegans] sp|Q23270|TRM1_CAEEL
            N(2),N(2)-dimethylguanosine tRNA methyltransferase
            (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
            2,2-dimethylguanosine-26 methyltransferase) (tRNA(m(2, mRNA
            sequence.
ACCESSION   EY190477
VERSION     EY190477.1  GI:159492921
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 54
            original value before phred recall for SR was 451
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttctat cagggggttt tttttttttt ttttgttgtt aggccctctt
       61 ttgggggggg cctcaacaaa ataaagtttg agaattgggg aagaatcact tcacaagggc
      121 ggccgtattt tagctcaccg atcctttggc cttggccttc ggtccccagt tttttccctt
      181 attatccgga aagcgaacaa gctggaccgc tttgctttga ggaacggcgt ccggatgcag
      241 cccgaagttt atttcgaatc tgggttcctg actcaaaatg cgccttccgg ccgactctga
      301 tccaagtttg tccgcgtcca cccctgactt ctttgcccac acacggcaga tgtcccacag
      361 aaatgtgagt ggcgcgtcag tcttaatcgc ctttcccggc cgta
//