LOCUS       EY190475                 344 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa01g10.b1 R.similis_EST_RSAB Radopholus similis cDNA 3'
            similar to ref|NP_502288.1| Emp24 gp25L p24 family [Caenorhabditis
            elegans] pir|T23466 hypothetical protein K08E4.6 - Caenorhabditis
            elegans emb|CAA92683.1| Hypothetical protein K08E4.6
            [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY190475
VERSION     EY190475.1  GI:159492919
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 882 was extended to value 883 (,)
            putative full insert read: vector-to-vector length is 721 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 52
            original value before phred recall for SR was 773
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 tttcaacatc acatgcgtta gccagtagtg taaaccatca aaacaaaggg cgaaatgtat
       61 tttagggaac cagttgtttg ggatagcgac cgaaaaggaa aggcgatgga agtggggatg
      121 cataagcgaa ggtggaaaat tcagtcattt gaaacatgct tcttcttcgt tttgtatcga
      181 taatttactt caccgtcatc tgaaagttca ccgccttcct ccccggatca tgcggcccag
      241 tttggagttc tcctcaaaca gactccgaat caaccacacc tgcagtacgc ccacagccag
      301 catcaccagt gtgttgatca acgaccatag ctgcacccga ttgt
//