LOCUS       EY190469                 447 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa01h06.b1 R.similis_EST_RSAB Radopholus similis cDNA 3'
            similar to ref|NP_506352.1| Transthyretin-like family member
            precursor family member (17.8 kD) [Caenorhabditis elegans]
            pir|T20267 hypothetical protein C56A3.2 - Caenorhabditis elegans
            emb|CAB01136.1| Hypothetical protein C56A3.2 [Caenorhabditis
            elegans], mRNA sequence.
ACCESSION   EY190469
VERSION     EY190469.1  GI:159492913
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 55
            original value before phred recall for SR was 500
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgtcccat aagggggttc tttttttttt tttctttaat gggcaacctt
       61 ttgggggcaa atcaattaaa aagcaatttg acaagtcgaa agagggtcga tttgtagtgt
      121 tttgtttgtt ttggcttatc cattatccaa attcttcttt ctttgccacc tagagacgaa
      181 cgaaaaagtt gaatttctcg ccgccccggt agtggtgcaa gtgtcgcctt ttcttgctca
      241 cgatcaactc cctctcctcg ttggcgaaga tggtctccag attcagcgtg ccgatgtcaa
      301 atgttttttt gggggtggct ccctcactga cgtacgagtt gggaatcttg aacttgattt
      361 tgcgagagcc aggcttaatc ccatcgtcgc agtcgtggta gatcttgaac acgacgtcga
      421 tgttggttgc ttcggtgccc ggccgta
//