LOCUS       EY190465                 354 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa01b12.b1 R.similis_EST_RSAB Radopholus similis cDNA 3',
            mRNA sequence.
ACCESSION   EY190465
VERSION     EY190465.1  GI:159492909
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 816 was extended to value 816 (,)
            putative full insert read: vector-to-vector length is 529 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 48
            original value before phred recall for SR was 577
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttcttt ttggggtttt tttttttttt ttttgctgtg gagcaacttt
       61 ttttgtctgc aataaccttt atatggaaga ttgttttgaa cgctcttatt tgataaaggg
      121 aaattatgtt ttgcgtgact gacgagtatc aatcctactg cccaacgaaa ccagccaaaa
      181 gtttctccaa ggattacgct tccttagacg gaccggtggt gcgaccttct tccccctcca
      241 atttttttaa tcccaccaat ggttccttgc ccttccacat ttcgaattac ctcttttgct
      301 ggaacaagaa ttgcccactt ttaaatactc catgggggat ttaaacctct tccc
//