LOCUS EY190462 301 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAB-aaa01f05.b1 R.similis_EST_RSAB Radopholus similis cDNA 3', mRNA sequence. ACCESSION EY190462 VERSION EY190462.1 GI:159492906 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 301) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 906 was extended to value 906 (,) This trace has been recalled with phred original value before phred recall for SL was 51 original value before phred recall for SR was 349 Seq primer: pDNR-LIB-reverse. FEATURES Location/Qualifiers source 1..301 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022112 R.similis_EST_RSAB" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 aggcggccga catgttcttt tagggggttt tttttttttt ttttacacca tgctcccctt 61 ggaggcggaa gactttcgtt tccgggacgc tccccaccag ggcgaaatgc tccggcggcg 121 gatcgcgatt tggcatcgtt tacggtcaga actagggcgg tatctgatcg ccttcgatcc 181 tctgactttc gttcttgatt aatgaagaca ttcttggcaa gtgctttcgc tgtagtttgt 241 cttgcggcgg tccaagaatt tcacctctca cgccgcaata caaatgcccc ccccggccgt 301 a //