LOCUS       EY190442                 605 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa01d10.b1 R.similis_EST_RSAB Radopholus similis cDNA 3'
            similar to ref|NP_505945.1| Protease inhibitor family member (24.4
            kD) [Caenorhabditis elegans] pir|T21736 hypothetical protein
            F35B12.6 - Caenorhabditis elegans emb|CAA98467.1| Hypothetical
            protein F35B12.6 [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY190442
VERSION     EY190442.1  GI:159492886
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 763 (,)
            original QR value of 869 was extended to value 870 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 56
            original value before phred recall for SR was 819
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgtcctat tgaggggttt tttttttttt ttttcttttt ttttgccctt
       61 tggggggctt tatacattgt tttgtaagac atattggtcg attccgatga tgccggttcc
      121 tgcggttcct tcccattcct cggcgtccat cagacgccct tgcaatattt gatgcagtcg
      181 tagtacgatt ggaagttgtt ggcgttgccc agagccccgt agaaggtgaa tatctggcag
      241 tcgccgtccg cgctcttgta gaagtagtgc ggcgtgtgtc tgcccatgaa ggcgtacctg
      301 ccggtgtcgt ggtccacctt gcagaccacg tctttgttcg ccggacagca cttccccccg
      361 gtgcacttgt agccgggggg acactggtcc ccgggacagt gcggcaccct cgcctccgcg
      421 tcaactgcag agcgcactcc gccctcgcac atgggcactg ggagcaccat gctggacttc
      481 ttctcctcca ccttgaagcg gccgcacctt ttctggcact ccgccctcgt gccgaatcgg
      541 ttctcggtgc cgccgtagcc caggtaggca aatggctggc accgcttggt caccgtgtcg
      601 aagaa
//