LOCUS EY190435 600 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAB-aaa01f04.b1 R.similis_EST_RSAB Radopholus similis cDNA 3' similar to ref|NP_509658.1| Multidrug resistance-associated protein [Caenorhabditis elegans] pir|T21219 hypothetical protein F21G4.2 - Caenorhabditis elegans emb|CAB02667.1| C. elegans MRP-4 protein (corresponding sequence F21G4.2) [Caenorhabditis elegans], mRNA sequence. ACCESSION EY190435 VERSION EY190435.1 GI:159492879 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 600) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 902 was extended to value 902 (,) This trace has been recalled with phred original value before phred recall for SL was 56 original value before phred recall for SR was 883 COMM possible reversed clone; protein similarity on negative strand Seq primer: pDNR-LIB-reverse. FEATURES Location/Qualifiers source 1..600 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022112 R.similis_EST_RSAB" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 aggcggccga catgtccctt taagggtttt tttttttttt tttgttagag tggaatcctt 61 ttatttgtag taaaataggt ccattgaaaa gcaacaataa cagttagagg aggtgggcag 121 gttgtcaagt atatgcgcat ttaggcagtc aatgtcggcc gtctcaactt atgctcggct 181 gctcgctatt tgagttgacc aattcgaaga attgggaatg tttgtttgcc atcaggttgg 241 taggtgtgtc aaactcgaca agactgccac tggatagcac cataattcga tcgtagtcca 301 gtacagtgct aatgcggtgt gcaatcgtta gaatggtgga gtgctgaaac tcggaacgga 361 ttgttttttg gatgagcgtg tctgtttgtg catcaataga tgctgtagct tcgtccagca 421 caatcacggc acttctgcgc agaatggcac gtgccagaca caggagttga cgctgtccca 481 cactgatgtt gtccccactc tccgcgatct ggtgagatag gccttcatgt tgttcccgta 541 caaactcctt cagatggacg tgctcaaggg ccatccacag ttccgcatcg gtgtatacgt //