LOCUS       EY190427                 454 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa01c04.b1 R.similis_EST_RSAB Radopholus similis cDNA 3'
            similar to gb|AAD43464.1| pre-mRNA splicing factor [Heterodera
            glycines], mRNA sequence.
ACCESSION   EY190427
VERSION     EY190427.1  GI:159492871
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 871 was extended to value 872 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 54
            original value before phred recall for SR was 502
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgtcccct cagggggttt tttttttttt ttttgacatt tagggtccct
       61 ttggggggga aaaatagcaa atggaggata aaaattggct tggtcatgtg attaaaaatc
      121 cccaattctt tttcagaacc acatcttgat agtgctgtcc cagccgcacg aaatcatgcg
      181 tgatttctcg tggggatgcc acagcactga gatgcacacg ttgtcgtgtg ccttccaagt
      241 ggacacaatc ttgtgggtgc gccagtccca aatcgtgatt ttcccgtccg cgtctccaga
      301 ggacaaaaag ctcatctccg gcgagaaatc cgttgagcac gcgtatcctg cagtgttgtg
      361 cccgcggaag gcctttttgc gagcgaaacg cagtttgtcg tccacaattt ggaagaggac
      421 aattctgttg tccatcgact ggcccccggc cgta
//