LOCUS       EY190409                 284 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa04g09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190409
VERSION     EY190409.1  GI:159493012
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 917 was extended to value 918 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 156
            original value before phred recall for SR was 485
            PolyA tail found inside read...SR being truncated to 450
            Potential full insert read...vec-to-vec length: 321
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggcacc cttgcatttt ttgcctttct acgtcgttcg gagagaccca ccatccacca
       61 cctttgttgt tcccatccac acccaaccgc cacaacccaa gatccatccc cctcaccctc
      121 ctgttgccgc tccctccgtc cctggcctag ccgcgtagag gggggagtcg gcccaattcg
      181 gcggcggatg atgtccacac tgccatccat tgtgcgcgtt gtttgtgttt tggggacact
      241 ctttgccgtc gtcacgtttc aaaggataaa cccgattatc tgca
//