LOCUS EY190408 464 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa04g07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190408 VERSION EY190408.1 GI:159493011 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 464) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 862 was extended to value 862 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 668 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..464 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtcc ctgtgcatgc gaaacatctc gctgctgcag cgaccgctct tgccgataaa 61 ttagcgttct acgagaatgc caatccaccg atagacatac ttctgaccga atcggattgt 121 gagcgcgagc tggccgaact tgcacagaac aagggtggtc tggctgtctc cagcgaatgg 181 gttattcaag caatcgtgac agggacattg ccgcagctta actgtgtggg aggggagcga 241 ttccgctatg attttgtttg aatattgaat cgataaagat acaaatttga cataaacaac 301 catcattctt atttttttat accttgcttc gtgtccaaac tcaagaattt ccgatgtatc 361 gatcctgctc gctcttctat caccttcatc aattgtcaat tttttgaaga tccaacaatt 421 gacgttgctt tgaagcaatt atgatcttcg tttatattta cttg //