LOCUS EY190406 418 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa04g05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_741598.1| Lifeguard (33.2 kD) [Caenorhabditis elegans] emb|CAD44126.1| Hypothetical protein F40F9.1b [Caenorhabditis elegans], mRNA sequence. ACCESSION EY190406 VERSION EY190406.1 GI:159493009 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 418) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 908 was extended to value 909 (,) This trace has been recalled with phred original value before phred recall for SL was 152 original value before phred recall for SR was 663 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..418 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggac cgtgtgtctg acgttgttct ctcgggggat catcattgtg ggcatgttca 61 ccaacttgcg cattgtgtac gtggtgtatg cggcgatcgg cgcattcctg tatatgatat 121 ggctggccat ccacattcag atggtgatgg gagggaagac ctatgacctt tctccggagg 181 agcacatatt ctcgtctatc accatctttt tggacatcat ctcagatctt ctggttactg 241 cttatgattt tcagcgacag aagctgaatt cagggatagt tgaattgacg aatcgaaacc 301 gctaaccagt gacatgggcg aatcagaagt aaatgcagtc tacaccatat atgatacatt 361 cgatccgatc tggcgatctt cattgtccta tccctgtgtg tatccagtaa tatttact //