LOCUS       EY190399                 468 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa04f04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_495781.1| Putative protein, nematode specific
            [Caenorhabditis elegans] pir|T21240 hypothetical protein F22B5.4 -
            Caenorhabditis elegans emb|CAA90356.1| Hypothetical protein F22B5.4
            [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY190399
VERSION     EY190399.1  GI:159493002
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 862 was extended to value 862 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 725
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcat gaagtatgaa cgtgactttt cacgcgaatc acgttataca aagccacata
       61 agaagaccga cacatttata agatttgtaa catttaacgc tcgccatcac atggagctct
      121 ggccgatcgt atttctctgc ggtgtctctt gttgcctagt cgtgggcata gcctacataa
      181 gttttgaaaa agtggaagtc tggcttgaca gaagcaccaa gaccccacca tgggattggg
      241 cgcgcattcg tgacaaatac tggagaatgc aaacagtatt tttcgatcca cttggcggga
      301 cccaccagcg tatagaaatc atggagaagc tacaggatga gatgttggag acggccaagc
      361 agcgtggaac ccgcgatgcc ggtggctaca ttgttactaa ctagaacggg aaggccagca
      421 aagcagatgt aaagatgaaa cgaatgacca gagactaatt gaccattc
//