LOCUS EY190389 213 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa04e06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190389 VERSION EY190389.1 GI:159492992 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 213) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 919 was extended to value 919 (,) This trace has been recalled with phred original value before phred recall for SL was 158 original value before phred recall for SR was 415 PolyA tail found inside read...SR being truncated to 379 Potential full insert read...vec-to-vec length: 351 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..213 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcat cttggaccga ttctgctgct gcggaggcac ctccccggac ttctaaggcg 61 cgcaacacac atgccctagt cacctgcacg gtcggcttgc attcgactcc ctccctgacc 121 ctagcctctg attaaaaaga aacgtgtaat caataacctg tcactttgcc gatgatgcac 181 ttttgccgaa taaatcacgt gactgactaa aga //