LOCUS EY190380 189 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa04d09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190380 VERSION EY190380.1 GI:159492983 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 189) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 868 was extended to value 869 (,) This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 412 PolyA tail found inside read...SR being truncated to 341 Potential full insert read...vec-to-vec length: 261 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..189 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtga tgaatcgaat tgcagagctg cattcttaaa ttgcattaaa gtaccacaat 61 cgataaccgc agttgattta ttcattccta accctgatta agtatatttc tcgattttgt 121 ttccgaatca tgcatattat ttgtcaaacc atttattcgg cctgtagaaa taaagtcatt 181 gtattttga //