LOCUS       EY190378                 327 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa04d06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190378
VERSION     EY190378.1  GI:159492981
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 903 was extended to value 903 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 156
            original value before phred recall for SR was 528
            PolyA tail found inside read...SR being truncated to 492
            Potential full insert read...vec-to-vec length: 468
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaca aggggggggc agtcacgctt caagaaatgg agagcttcct gggcggcata
       61 tacaaaatgc tgggcgacgc actgcagctc tcatcggagg cggagggaac gcccgagagg
      121 cggacggcgc tgatctgggc cacgttgggg aaggaggcgg acggcaagtt gtcgctggac
      181 gagttcacgg aaagtgccaa gtcggatgcg aacattgttc aggcattcag tctctacgag
      241 gggctgggca agtgacagac ccccaagcaa tcaaccgcac ataatcaacc gcattccttt
      301 gtcgaataat aaactggcgc aatcaca
//