LOCUS       EY190368                 258 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa04c08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_536715.2| ATPase, H+ transporting, lysosomal V0
            subunit A isoform 4; ATPase, H+ transporting, lysosomal (vacuolar
            proton pump) noncatalytic accessory protein 1B; V-ATPase alpha 4
            [Mus musculus] gb|AAN45855.1|AF435090_1 vacuolar proton translo,
            mRNA sequence.
ACCESSION   EY190368
VERSION     EY190368.1  GI:159492971
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 904 was extended to value 905 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 157
            original value before phred recall for SR was 461
            PolyA tail found inside read...SR being truncated to 423
            Potential full insert read...vec-to-vec length: 399
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggtg gcgttctttg tcttcttctt actgatggtt gtgattctgg tcgtgatgga
       61 aggtctctcc gcgttcttgc acgcccttcg tctgcattgg gttgagttcc aaagcaagtt
      121 ctacgagggc tgtggaaatg cattcgcccc gttcaacttt gtcaactcac tcaaaaaatt
      181 gtgttccgaa tcgatcgtat actgattttt tacaacttgt tgtgaatgaa tctatatttg
      241 ataaaatgat agttggga
//