LOCUS EY190361 402 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa04b11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_492582.1| Caax prenyl protease (50.7 kD) [Caenorhabditis elegans] pir|T18917 hypothetical protein C04F12.10 - Caenorhabditis elegans emb|CAB03839.1| Hypothetical protein C04F12.10 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY190361 VERSION EY190361.1 GI:159492964 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 402) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 161 original value before phred recall for SR was 635 PolyA tail found inside read...SR being truncated to 597 Potential full insert read...vec-to-vec length: 401 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..402 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcaa caacttttct ttgcatttgg gtttcccccc ggcgcacaac ccgcagtcat 61 cggactggtg atcatactcc agtatgtgat ggcgccatac aatgacgtac tctctgtgct 121 gatgaatgtg ctcagccgga agatggaatt tgctgccgac agattctctg caaatttggg 181 gtgcagcgag gaactgatca gtggactcgt gaaactaggc aatgacaacc tctcgatgcc 241 aatcgatgac tggctctatt cctggtgcaa ccactcgcac cctccagtcc cagaaagaat 301 tgaagcgctc aagaaatacg aatagcgaat acatatgtcg gtttttagtt ttttcaaccg 361 taaattctga gttaacaaat gtgatgaatt gattcgctct aa //