LOCUS EY190355 197 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa04b05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190355 VERSION EY190355.1 GI:159492958 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 197) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 915 was extended to value 916 (,) This trace has been recalled with phred original value before phred recall for SL was 153 original value before phred recall for SR was 395 PolyA tail found inside read...SR being truncated to 351 Potential full insert read...vec-to-vec length: 335 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..197 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcaa cggaagggcg ctggccaccc atcccgcgct tgctcgacac ttcgatgagc 61 atgatcatcc gaacagggcg aggacagcgc tcacgcaatt cgtcatacga tgattaacaa 121 aatgaatgaa tgaaatgcga atgtcatatt tattgtactt tatttggatt gcctatttgt 181 aaaaatgagc ttggaga //