LOCUS EY190339 399 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa03h09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_498572.1| Ribosomal Protein, Small subunit RPS-14 (16.2 kD) (rps-14) [Caenorhabditis elegans] sp|P48150|RS14_CAEEL 40S ribosomal protein S14 pir|T28833 hypothetical protein F37C12.9 - Caenorhabditis elegans gb|AAC48301.1| Ribosomal protein, mRNA sequence. ACCESSION EY190339 VERSION EY190339.1 GI:159492942 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 399) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 870 was extended to value 871 (,) This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 593 PolyA tail found inside read...SR being truncated to 554 Potential full insert read...vec-to-vec length: 544 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..399 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtc cgggcgcgag acaatcgtca agatcaccgg tggaatgcga gtgaaggccg 61 accgagacga agccagtcct tatgcggcca tgcttgctgc gcaagatgtg gcggagcgct 121 gtaaacaggt gggggttaat gccctgcacg tcaaactgcg tgccactggg ggtacaaaga 181 ccaagacgcc aggaccaggc gcccaggcag cgcttcgtgc tcttgctcgt tccggcatga 241 agatcggtcg gatcgaggat gtgacgccga ttccgactga caccactcgc cgaaagggcg 301 gacgtcgtgg acgccgtctc tgagcgctga gaattgtgga ggagttacag caatgttatt 361 gttattttcg aattgatttt cgaataaaac gtgcttgta //