LOCUS       EY190324                 379 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03g05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190324
VERSION     EY190324.1  GI:159493079
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 876 was extended to value 876 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 573
            PolyA tail found inside read...SR being truncated to 532
            Potential full insert read...vec-to-vec length: 520
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcga ggactagagt gcggctaatt ctctgttcag agggggatgc tgttaactgc
       61 gaaagggatg tgccgttgca tttgattcaa tcatttcttt gattctttct ctctgttttg
      121 ctccacaaca tacacacata ttattcggtc tgagcgcgtg tactcttatt tttttgttct
      181 tccaaacaca cacatttctt ctcaaggctt ggtcgcataa atgcgaaaat ttgaatcggc
      241 ctctctcttt tgaatgtaag taaaagataa cagagcatga tatgtatgtt aattgtaaat
      301 caccaatttc tttgttcttc cttccacaca catgaaacat gattatgaga agagaacgct
      361 ataaagatga aatatcaca
//