LOCUS       EY190315                 434 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03f07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190315
VERSION     EY190315.1  GI:159493070
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 888 was extended to value 888 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 634
            PolyA tail found inside read...SR being truncated to 586
            Potential full insert read...vec-to-vec length: 572
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat attgtattgt attggcattt tattttttct tattaaaggt tataatattt
       61 taacttttat tttatttaag aatagtaatt ttccaaatta aaggtttatt aagtttaatt
      121 ttttgtaatt atgattttag gttataaata attaatgaag tataaactat taatgttata
      181 ttttttagat aaattaattt ttctagtaat ttatttttag ataaattaat gttccagaaa
      241 tatcggctag acattattat ttttaactaa aaacttttta aattttattt taatttatat
      301 aaatttatat taatataggt gaaattttaa ttataatcat gttaataatt ttataaaatt
      361 taaaatttta atttttaact taggttagac actaattaat gaaatttaat aattttcttt
      421 agtaaatttt tgca
//