LOCUS       EY190309                 328 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03f01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190309
VERSION     EY190309.1  GI:159493064
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 846 was extended to value 846 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 154
            original value before phred recall for SR was 527
            PolyA tail found inside read...SR being truncated to 482
            Potential full insert read...vec-to-vec length: 445
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggag tgctgttcca tttctattcc cccactctca cgttgtctcc cgtttcgatc
       61 atttatgcgc ggggacgcgc attcggggag ccacgggaat gttatgggaa caaagtccat
      121 gagtaggagc aaacgtcaat gggggccatg gctagggcca agccgttaag cctcccataa
      181 atcattgtgg cccaccaatg ctctggggag aaggcatctt ctttaaagcc cttccctttt
      241 ctattcttgg ggctcactct ctatatcgcc caaccatcag ccgatgttac ggagtgacga
      301 accagcagaa taaaggattg aaccctca
//