LOCUS       EY190308                 370 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03e11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190308
VERSION     EY190308.1  GI:159493063
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 831 was extended to value 832 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 160
            original value before phred recall for SR was 575
            PolyA tail found inside read...SR being truncated to 537
            Potential full insert read...vec-to-vec length: 402
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc cggaacgcta ccttcaccta ctcgagtccc tatgtgctct atcggaccac
       61 acgcgagatt gccttcaaca actcggtgga actgaccgga tacacagccg agtactgcca
      121 ggcaccgttc tcgcgtctga ccagcctgta catcagtctg cagttcgatg tcacggccac
      181 gctgcgctac tactacgggc accaggagca ggtcactctg tccatgcagc agcaggtgtg
      241 ctgtgtgccc agcggaaact gcacctccgg atgggtggac ctctgaaatg gagaaggaag
      301 agatttcact ttttgcgcta gtcttggcat actaatcaag catgacttcg gatcaaatgt
      361 cagaaaagga
//