LOCUS       EY190291                 250 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03d05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190291
VERSION     EY190291.1  GI:159493046
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 920 was extended to value 921 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 160
            original value before phred recall for SR was 454
            PolyA tail found inside read...SR being truncated to 414
            Potential full insert read...vec-to-vec length: 367
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc cctctgcgtc aagtcggttt aagtggaggg ccgtccggtg gagtcctatc
       61 tgttcatgtt ccggctgcgg aatgcccagg tgctgctgga ggacctgcgt ttggagggcg
      121 ccttcaagtt cgagggactg gtcgtgtact gagtgagggt cgggtagact tggcgtattt
      181 ggattgagtt tcgcgcaatt caatcaaatt ccacaacttt tttgcaaaac aaaggaagga
      241 ctccctacga
//