LOCUS       EY190288                 272 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03d02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_510427.1| Putative protein of eukaryotic origin
            [Caenorhabditis elegans] emb|CAB01868.2| Hypothetical protein
            C44H4.4 [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY190288
VERSION     EY190288.1  GI:159493043
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 872 was extended to value 873 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 467
            PolyA tail found inside read...SR being truncated to 424
            Potential full insert read...vec-to-vec length: 306
            COMM   possible reversed clone; protein similarity on positive
            strand
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcgc cacggaatat tcacacgcac ccttaattat agattagtta cttcagcctc
       61 tcgattttaa tttgaacaaa cctgtcacag gaggcgctca gaatagtcag ccacggcggc
      121 aactccaatt tctccgccaa aacgtcttca ttgagctcca aattgcgcag atgagccttt
      181 ccccgcaaaa cttccattgt aatctgtatc tggcttcaaa tttcgagcaa acctgaatat
      241 ttttgttgaa tgctagataa attaatgaca ta
//