LOCUS       EY190282                 545 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03c08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_492823.1| Putative plasma membrane membrane
            protein, with at least 14 transmembrane domains, 2 coiled coil-4
            domains, of eukaryotic origin [Caenorhabditis elegans]
            gb|AAF39833.1| Hypothetical protein F55A3.1 [Caenorhabditis
            elegans], mRNA sequence.
ACCESSION   EY190282
VERSION     EY190282.1  GI:159493037
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 863
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggagg atgggcctac atgaaaaatg tcttcaattc tgcggcctgg ctgattttgc
       61 gtgtggtcgc tgcttccttc ccactactgg tcgtaattcc attcatgctc ggcctctact
      121 tccagctgct tgtgattagt ccactgcgtg tgacgatttt ccaatcgccg ttgtttttcc
      181 cctggaagga atgggccatg ggagtagtgc actttaaaat tctgtgcgca tccgtgctta
      241 tgggtcccga ttggtggatg aagacggctt ttgaacagat ctacgctgac gggatctaga
      301 acttccgact ccgtgacctc tacgtgaact tggtcttgcc gattgtgaat gcacttgcat
      361 ttctcatcgc cttgccatac accttcagca agttgttgct gatcttcatc gaatcttccc
      421 gagaagaaca agtaatcgtt acgcgatatg catatccctg tatgctcgga tccgtctgca
      481 tttgcgcatt cctcacctgg cagtggcaaa agttgaaact gctggcgcag aaaatccgca
      541 atgac
//