LOCUS       EY190280                 464 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03c06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190280
VERSION     EY190280.1  GI:159493035
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 893 was extended to value 894 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 156
            original value before phred recall for SR was 665
            PolyA tail found inside read...SR being truncated to 629
            Potential full insert read...vec-to-vec length: 582
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggta cgaaaagctg ggcaagaatc acaccatgga gcaggcgatc aaggcggagt
       61 tcagcggaga cgatctgaac gcccttctgt ccatctgcag ctttgtgcgc aacggcatca
      121 tcggcgaggt ggcggaactg ctgcacaagt gcctcgtgaa gggcggcaaa gacgacctgc
      181 tgatccacct ggtcatctcg cactcggagc tggatttgca ggacatagag aacgagtacc
      241 tcaagcggtt caagattccg ctcgagcagg ccaccgcgga cagcggaaag ccctcaactc
      301 tgaagaatgc gctgatccgc atgatcaagg gcaacaagta gtccgactgg ggatgcctta
      361 cttcatattt ggctgagacc gatacgctat tttgtcgcat tttagaacaa attgttcact
      421 catagaatta atgttaataa tctggcaaaa taatgttttt acca
//