LOCUS       EY190278                 472 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03c04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190278
VERSION     EY190278.1  GI:159493033
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 885 was extended to value 885 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 147
            original value before phred recall for SR was 664
            PolyA tail found inside read...SR being truncated to 624
            Potential full insert read...vec-to-vec length: 614
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaca catgttccgt actccactcc tgtgtacagg tatcttcttt gagcgatgcg
       61 cggtacacaa atccatccat cagcgaagat atattgattt accgatttcc tgcctattct
      121 attgtagtat agatgtatag aaattagtag aaagcagtgg aagttgtata gaattctaaa
      181 aagtattaag taatttacac atttagtgtt cggacaatta atcccctcgc tttagtgtta
      241 cccagccagt acccaatcaa tccgatccca attctcaggc caaatcactc acaaagccaa
      301 tattcagtat acaccaatca gccaatcacc acattctcac aaaatgtgtt gtaaatatca
      361 tttcaatgtg ttttcaaaac caccggcctc ttttgcattt gtaaagcacc agaaatttag
      421 tgtgtgtggg atcaacgttt ctcaacattt tggctgataa acttgcgtaa ta
//