LOCUS EY190271 238 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa03b09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190271 VERSION EY190271.1 GI:159493026 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 238) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 879 was extended to value 879 (,) This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 435 PolyA tail found inside read...SR being truncated to 392 Potential full insert read...vec-to-vec length: 379 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..238 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcag tcagaagtgg gagcttctcc gcaggggagg gcgtgccggt ggagacagcc 61 gatgtgcaag tggccgttgg ggagggacaa ccgcaaggac tgagttgccc gtcgagcgcg 121 acatctcaac aagtgggatc tcatcccgga ggagccggat atcaatgcaa catttgtggt 181 cgaagcttcc caaagaaaaa gggcatcgca atacacaatg cgtttgcgca caacatta //