LOCUS EY190267 232 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa03b05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190267 VERSION EY190267.1 GI:159493022 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 232) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 727 was extended to value 727 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 730 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..232 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 gcatcaaaaa tttaacgcaa aaaataaatt gatgatgtga aaaacacata ttccatgatg 61 aattgatgaa tgtggaaatg cgagtaaaag ttatgttgtg gtaaaatgat ttgcagcaaa 121 acaattcaaa cgcgggagat ggatcagtgg aagaatttgc aattgcaaac tttggaccag 181 gaaattactt tgagttggag cgacaattgc ggaaggaaca cattgcaaaa ag //