LOCUS       EY190266                 273 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03b04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190266
VERSION     EY190266.1  GI:159493021
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 885 was extended to value 885 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 469
            PolyA tail found inside read...SR being truncated to 425
            Potential full insert read...vec-to-vec length: 306
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtac acgcgggaga ctacagtgat catccagatc gcgactatta gcatcttctg
       61 tggctgcaat tgaggcgcaa gacagaagag agtggcgtcc aacggattcc tttctcgagg
      121 aagtcaccac gtgttgagcc gcaccttttc tctccagcac tttcctgtgc atagtcctaa
      181 aagaggcaag acatcgccct ttgacccaaa acgacagaac accgccgctt tttgtagttt
      241 tcttacactt tccttgtgaa taaatggtgc gta
//