LOCUS EY190264 217 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa03b02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190264 VERSION EY190264.1 GI:159493019 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 217) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 874 was extended to value 875 (,) This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 413 PolyA tail found inside read...SR being truncated to 370 Potential full insert read...vec-to-vec length: 355 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..217 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcaa gtaggtgggc gtggacccca agatggacaa ccgactctac gccaaccaga 61 aggaggcgag cggcgcaact gtgttcgcga tggaccattg agaagagaag ttaagaagaa 121 aactgctaat ggagcgaaaa atgaagtgat cgcccgattg acaatgatcg cacaatcttc 181 acatatattc aatgcgaatg gataaaaata tggcaca //