LOCUS EY190252 240 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa02h10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190252 VERSION EY190252.1 GI:159492567 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 240) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 871 was extended to value 872 (,) This trace has been recalled with phred original value before phred recall for SL was 158 original value before phred recall for SR was 442 PolyA tail found inside read...SR being truncated to 406 Potential full insert read...vec-to-vec length: 378 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..240 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtc gccgcgaccc acaatcgtgg ggctgctgca cctactgctg ttgaacacgg 61 tgctggcctc cgccctgttg gaggatgggc ggagagaact gccgagggat ggatggacga 121 tgggcacctg agaacgcata acacccaata aatagtaata atgggtccga cactgtgata 181 gccatattca gcattttcaa cattagtcaa aatgcccatt tccaataaat caaaacatta //