LOCUS       EY190250                 513 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02h08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190250
VERSION     EY190250.1  GI:159492565
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 865 was extended to value 866 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 159
            original value before phred recall for SR was 739
            PolyA tail found inside read...SR being truncated to 677
            Potential full insert read...vec-to-vec length: 630
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac tgaaaaaagg gggagacttg tgttctgggc caaatcgttg gagggacttc
       61 gcgcaatggt tccgctgcat gttgcagata aattttctcg gaaactagat ctcaacattc
      121 cacgacaaat cgaactccca gcaaagatcg aaatcatggc tcccccaata acgcatgagg
      181 acattgcgac aatgacattt ttgtccgcat tgcacgcatt tgaacaaaac ggaaccgaag
      241 taactggtca gcatgcgacc agaaagcagc ttgaagagaa tccattcgat gcaatgaaat
      301 tcacgcaacc acttgtacag atacccatga tcacggaaca agacgtaacg ctccaggaga
      361 ggcgcgttct agacgcgagg aaaagattgc aagaactcag aaaatccttt ttgatacgtt
      421 aaatcatccg attcaaacac aataatttca acttaatgca cgacttgtca tgaagatgta
      481 actgaagttt tgcatataaa atatccagta tga
//