LOCUS       EY190239                 354 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02g09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190239
VERSION     EY190239.1  GI:159492554
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 874 was extended to value 875 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 549
            PolyA tail found inside read...SR being truncated to 505
            Potential full insert read...vec-to-vec length: 494
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga acatcgctta ttccaggttt gattccctgc gcgaggattg gctggcgcat
       61 aaagaggctg tcatggccga gtggctagtc aatttgacag caagaatgga caacagcttc
      121 gcgtcgcatt ctcctgcgct ccaaatgtcg cgattaattt gcactttggt ggaattgaac
      181 gccgtcgctc tgagtttggc tgaagcaagc actgttctac gcatattcca tccggtcatc
      241 cacgagaaat gtccattaga acagggcaaa tgagaaatta cagacaaaaa aaaaacaaaa
      301 tattgagatg tgctctttac agaaacctaa aaatgtctta ataaacatat caca
//