LOCUS EY190238 306 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa02g08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190238 VERSION EY190238.1 GI:159492553 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 306) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 161 original value before phred recall for SR was 513 PolyA tail found inside read...SR being truncated to 472 Potential full insert read...vec-to-vec length: 382 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..306 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggta cgccggtgtg acgaccggtg ttgtgattct caacgatgcg gcttctagca 61 aaacggatcc gggaaagagg aaattcatgt cctgcgttgg catcggactt gtggtggtgc 121 tcttcagcct gctgctctct gtttttcgca gcaagtatca cggctacccg taccagttcc 181 tttttagcta gaagcacgaa aggagaagag tgtgatgggc aaggaaacag tcccgaaaat 241 tcgtttaaaa tacccctaat tatcctgcgg ttttctttgt tcaaaagtaa taaatcttgg 301 ctttta //