LOCUS EY190234 361 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa02g04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_001021.1| ribosomal protein S27; metallopanstimulin 1; 40S ribosomal protein S27 [Homo sapiens] ref|NP_446049.1| ribosomal protein S27 [Rattus norvegicus] ref|NP_081291.1| ribosomal protein S27 [Mus musculus] sp|P42677|RS27_HUMAN 40S riboso, mRNA sequence. ACCESSION EY190234 VERSION EY190234.1 GI:159492549 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 361) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 880 was extended to value 881 (,) This trace has been recalled with phred original value before phred recall for SL was 143 original value before phred recall for SR was 550 PolyA tail found inside read...SR being truncated to 526 Potential full insert read...vec-to-vec length: 500 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..361 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggcg agagttcaca aaatgccctt ggcgcaagac ctactttacc ctgacccgca 61 aaaggagcga caaaagcaca agccgaagcg actcgtccag cacccgaatt cattcttcat 121 ggacgtcaag tgccctggct gctacaagat caccaccgtg ttcagccatg cgcagacagt 181 cgtggtgtgc gtcggatgca gcaccgtgct gtgccagccg accggcggaa aggcgcgtct 241 ggtggaggga tgctcgttca gaaagaagca gcactgaaca gaagatggac gtatctggtt 301 tgtcgaactg tttttgttga tcggtaattt ggacggcgcc acaatatata tttgttgtcg 361 a //