LOCUS       EY190233                 356 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02g03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190233
VERSION     EY190233.1  GI:159492548
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 887 was extended to value 892 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 550
            PolyA tail found inside read...SR being truncated to 507
            Potential full insert read...vec-to-vec length: 496
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa gtgcgccgga atgaaccgac caaatacgaa cgagcgctct ggaaagatgc
       61 agtcgagaca atcaaggata ttcacacaat caaagagcgc cgctacggac aacacattgc
      121 gaatacgctg aagccgggta aacaaattgc gaagagggca atgctgcaga aggcgagaaa
      181 gaaagtgcat ctgatcagag tgccagtggc agatgaccaa gaagaggaag aggtggagac
      241 tgaggaggat gaaggaaagc cggaactgga ggaaggtgaa gcgcagatgg aggtcaatta
      301 agcaaagtga ttttgtttct tgtttaacat ttccgcgata aatcgtaact ggtcga
//