LOCUS       EY190221                 368 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02f02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to gb|AAO43307.1| putative polyubiquitin [Arabidopsis
            thaliana], mRNA sequence.
ACCESSION   EY190221
VERSION     EY190221.1  GI:159492536
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 896 was extended to value 897 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 153
            original value before phred recall for SR was 568
            PolyA tail found inside read...SR being truncated to 520
            Potential full insert read...vec-to-vec length: 506
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtca aggccaagat ccaggacacg gaaggcattc cgccggacca gcagcgtctg
       61 atcttcgccg gaaagcagct cgaggacggt cgcacccttg cggactacaa catccagaag
      121 gaatccaccc tccatctggt gttgcgtctt cgcggtggtt ccgcctgagt cagagaacgc
      181 gcagaggaat atggcgtctt gagatgtgga ccaattttaa ttcctgttct gatcacacaa
      241 aacacaatcc acatgtttta cgatatacat aacagcagaa aacaacctac attcattaag
      301 taatcatgct ttctcgatat gtaattctga aatggctttc gcggaacagt aaagaataca
      361 aaatggca
//