LOCUS       EY190218                 407 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02e11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_497500.1| Phosphatidylinositol-4-phosphate
            5-kinase type II (45.5 kD) [Caenorhabditis elegans] gb|AAK29922.1|
            Hypothetical protein Y48G9A.8 [Caenorhabditis elegans], mRNA
            sequence.
ACCESSION   EY190218
VERSION     EY190218.1  GI:159492533
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 837 was extended to value 837 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 601
            PolyA tail found inside read...SR being truncated to 564
            Potential full insert read...vec-to-vec length: 544
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac tgaacctaga cgacgatttc tttgccattc cgtccagcga agtgtcaaag
       61 agaaaactgg tctatttcat tggtctggtc gacattttga cttattatgg agtgaagaag
      121 aggaccgaat ccgccgcaaa atctgcccta aggggctcag gagctgagcg agaaatttct
      181 accgttaaac cggagcagta tgcgaaacgg cttctggaat tcgtgaatcg taatgtggtt
      241 tcggctacac ttgtagaaga tacaattccg tctgaagtag cacaaattgc tattcactga
      301 tgaaaacaaa cgcagttatt actgaatact gatcgacaga atagagcttt ctccatttgc
      361 atcaaaatca aaaaatcaaa atgccatcaa taaactgtgc agggtca
//