LOCUS       EY190207                 499 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02d12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_509787.2| Putative nuclear protein, with a coiled
            coil domain, of bilaterial origin (21.3 kD) [Caenorhabditis
            elegans] sp|P55326|YZG1_CAEEL Hypothetical protein F13E6.1 in
            chromosome X emb|CAA92122.2| Hypothetical protein F13E6.1
            [Caenorhabd, mRNA sequence.
ACCESSION   EY190207
VERSION     EY190207.1  GI:159492522
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 816 was extended to value 817 (,)
            putative full insert read: vector-to-vector length is 620 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 159
            original value before phred recall for SR was 779
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc gcagggactc aaaactgtta aggaaacgac tgcatatcaa aagaccagtg
       61 aagttgttgc tgggacggct gagaccgtat caagcaaatt acacgacatt cgcaactcgt
      121 ccttcttcaa gtctttcgag tcgaagctcg gcaatgccat gatcactgcg aaaatggccg
      181 cttccacatc catcgaccac ttggctggtg ttgcgggcgg cactcgttcg cccactgcga
      241 gcactttgtc ggccaatcca gcagaggaca acaagagcgg ccagtcgccc ctctcgtgag
      301 tgcgcaaacg aaaatgagga cggacaaaag ttggtgtgct tcttgacttc cgcttcccaa
      361 ttgacttcca tcacccaaca gcagcgatta cctgtgttct gttgggctca gaaaacgaat
      421 atgcatctat ccattgttca tttctctctc tacttccttc accattcgat attctgttcg
      481 taatccattc gtgtaatga
//