LOCUS       EY190200                 527 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02d05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190200
VERSION     EY190200.1  GI:159492515
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 894 was extended to value 895 (,)
            putative full insert read: vector-to-vector length is 706 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 857
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcaa gcggaaccga gtctctgata gcgccaattg aggagcgagt gcattgtgtg
       61 caactgaacg acgacgacag cgaggaagaa atgcccgacc cggacattga gctggtggag
      121 gatagactca cactagccgg tccgacacag aaagaggtgg cgacaagtac ggcaagcaat
      181 gactgccaga gtgagcacca gacggagctg catgtcgcaa acgaacgtcc agtatcccta
      241 gaagacgaag aggcatcggc ggaggggcag ttgggcggcg aaggggaaaa ggaggcgagc
      301 gccgtccggc aagaagatga gagcgaaacg gcgagcaagc tgatccgttc tttgtccatt
      361 agcaatctcg agagcagtcc ctagccctac tgcatcgcca cgtctcagtc gatttgtggg
      421 agctccgcca cttcggcagg cgttttagag gcagctttgc cctcttcctt tttccacaac
      481 atttaacctt tctttactaa caacataccc atgttttcaa atctccg
//