LOCUS       EY190198                 306 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02d03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190198
VERSION     EY190198.1  GI:159492513
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 926 was extended to value 927 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 157
            original value before phred recall for SR was 508
            PolyA tail found inside read...SR being truncated to 471
            Potential full insert read...vec-to-vec length: 338
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggca gtggtatcaa cgcagagttc ccattacggc cggggtcttg gagtcgggtt
       61 gtttgggatc gcagcccaaa gaaggtggta aacttcatct aagactgaat atgactacga
      121 gaccgatagc gaacaagtac tgtgaaggaa agtcgaaaag caccagaaga gagggatgaa
      181 gagattccac tgatggatga cgatccaatg gcatcgtttt tattgacttt ttgatcaaaa
      241 tccgttcggg atattttcgt tcttttggac ataaacttac tgctttgcgg acaagcagaa
      301 gaaaca
//