LOCUS       EY190190                 437 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02c07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_491792.1| Phenylalanyl (F) tRNA Synthetase FRS-1
            (62.7 kD) (frs-1) [Caenorhabditis elegans] pir|T28752
            phenylalanine-tRNA ligase (EC 6.1.1.20) beta chain T08B2.9
            [similarity] - Caenorhabditis elegans gb|AAG00015.1| Phenylalanyl
            (f) trna syn, mRNA sequence.
ACCESSION   EY190190
VERSION     EY190190.1  GI:159492505
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 904 was extended to value 904 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 157
            original value before phred recall for SR was 667
            PolyA tail found inside read...SR being truncated to 602
            Potential full insert read...vec-to-vec length: 576
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggtt cttccgaaaa tgcggcattg aaaatctccg cttcaagccc acctacaacc
       61 cgtacacaga gccctcgatg gagatattcg ccttccacta cgggctgaac aagtgggtgg
      121 agattggcaa ttcgggcatg ttcaggccgg aaatgctgct gccaatggga cttcctgagg
      181 atgtggctgt ggcgggattc gggctgtcgc tggagagacc gaccatgatc cggtacggca
      241 tttcgaacat tcgggagctg ttcggccaca aaatcaacct ggaaatggtc tacaagaacc
      301 ccctctgcag acttgacaaa aaggactgac aaattcgggc aaacagtgtg accctagttt
      361 tttaaatttg tatcagttta tgttgttgat atgcttttat aatttatatc gtaattgata
      421 tgccaaacac tgcttta
//